Optimization of media composition by using sugarcane molasses for polyhydoxyalkanoate production in Ralstonia Eutropha strain /
A study of polyhydroxyalkanoate (PHA) production was conducted using pre-treatment of sugarcane molasses as main substrate to obtain low-cost medium for fermentation process. Ralstonia eutropha strain was used to produce polyhydroxyalkanoate in the nutrient limitation conditions. The study involved...
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| Formato: | Tesis Libro |
| Lenguaje: | English |
| Publicado: |
Perlis, Malaysia
School of Bioprocess Engineering, University Malaysia Perlis
2013.
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| Sumario: | A study of polyhydroxyalkanoate (PHA) production was conducted using pre-treatment of sugarcane molasses as main substrate to obtain low-cost medium for fermentation process. Ralstonia eutropha strain was used to produce polyhydroxyalkanoate in the nutrient limitation conditions. The study involved determination of significance interaction between different parameter which is sugarcane molasses, ammonium sulfate and urea using Central Composite Design in order to optimize the PHA concentration. Two-stage fermentation techniques were used to obtained high yield of PHA fermentation. First stage fermentation involved bacteria growth phase in a nutrient rich medium and second stage fermentation involved culturing bacteria strain in the limited nutrient medium. The fermentation process was incubated at fixed set point values of agitation speed, pH, fermentation period and temperature. The highest PHA composition recorded was 72.03 % at 2 % sugarcane molasses, 0.21 % ammonium sulfate and 1 % urea concentrations. |
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| Descripción Física: | xi, 50 pages illustrations (some colour) 30 cm. |
| Bibliografía: | Includes bibliographical references (pages 36-43) |